FIG. 5.

Formation of [³H]PEt in J774 cells prelabeled with [9,10-³H]palmitate at 90 min after infection with L. monocytogenes strains. [³H]PEt was extracted from the cells and separated by thin-layer chromatography as described in the text. The data represent the means ± the SD of two separate experiments carried out in duplicate, in which each mutant strain was compared with the wild type in the same experiment. For wild type, n = 22; for all other strains, n = 4. The difference between the wild-type and the Δhly infections was highly significant (P < 0.0001, unpaired t test). The difference in [³H]PEt between uninfected control cells and the cells infected with the Δhly strain was not significant.