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[Preprint]. 2023 Apr 17:2023.04.17.536878. [Version 1] doi: 10.1101/2023.04.17.536878

Figure 2.

Figure 2.

Independent recombination of the two floxed alleles on a single cell level in the P2RY12CreER double reporter (Ai9:R26-YFP) mouse line. Evaluation of the TAM-dependent recombination of either Ai9-tdTomato or R26-YFP allele which are both located in the ROSA26 loci in a double reporter mouse in the P2RY12CreER line suggests that although on a populational level, Ai9-tdTomato reporter has a higher probability of being recombined than the R26-YFP allele, on a single cell level, the recombination of each individual allele can be independent and does not always follow the size of the floxed region rule. (A), experimental timeline. (B-E), IHC evaluation of the recombination of microglia on either of the reporter expression. Note tdTomato+:YFP+ double positive microglia (orange arrow), more abundant tdTomato+:YFP− microglia (white arrow) and the less abundant YFP+:tdTomato- microglia (white arrowhead). (F-G), representative FACS plots for no color control or the double reporter flow analysis. For quantification of % of each population, see Supplementary Table 1.