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[Preprint]. 2023 Apr 17:2023.04.17.536878. [Version 1] doi: 10.1101/2023.04.17.536878

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Figure 3. — Evaluation of the gene deletion efficiency on distinct homozygous floxed target gene alleles in the Cx3cr1CreER(Jung) and P2Ry12CreER drivers using quantitative RT-PCR. (A-D). Animal genotype and experimental flow. Total mRNA levels are evaluated for the floxed exon in the TGFb1 gene in YFP+ microglia sorted from the Cx3cr1^CreER(Jung) or P2RY12^CreER—TGFb1 ^fl/fl-R26-YFP mice at 3 weeks after TAM treatment. (E-G). Total mRNA levels are evaluated for the floxed exon in the ALK5 gene in either YFP+ microglia sorted from the Cx3cr1^CreER(Jung) or P2RY12^CreER—ALK5 ^fl/fl-R26-YFP mice or tdTomato+ microglia from the P2RY12^CreER—ALK5 ^fl/fl-Ai9 mice at 3 weeks after TAM treatment. Each data point represents the average of 1 animal (the average for each animal is obtained by averaging 3 technical replication of the qRT-PCR reaction for that animal) and the average for each animal was used as a single data point for statistical analysis. **p < 0.01 and ***p < 0.001, **** p<0.0001 for Two way ANOVA analysis, Tukey post-hoc pairwise analysis.