Figure 4. Proline-derived amino acids appear later in the retina than the RPE/Cho.

(A) A schematic of in vivo tracing of ¹⁵N proline. Mice were fasted for 18 hours and received a single injection of ¹⁵N proline (150 mg/kg) or PBS. Retina and RPE/Cho were harvested at 0min, 5min, 15min and 30min after injection for metabolite analysis with GC MS. (B-H) ¹⁵N fraction of proline and metabolites derived from ¹⁵N proline in RPE/Cho and retina at different time points. N=4.