Figure 2. FL2 protein is newly synthesized upon migration.

FL2 protein stain (red) and actin network stain (white) in U2OS cells incubated for one hour after a scratch. A) FL2 protein expression in untreated cells after a scratch. Sequence of images is a merged image of FL2 and actin, an image of the actin stain, an image of the FL2 stain and a zoomed in image of the cell edge indicated by white box in previous image. B) FL2 protein expression in cells treated with 200ug of puromycin prior to and during scratch. Sequence of images is the same as panel A. C) Quantification of the number of cells with significant FL2 protein expression at the leading edge. Untreated n=63; Puromycin n=63. Significant protein expression defined as fluorescence intensity 40% greater than the cytoplasm of the same cell and spanning at least a fourth of the cell edge.