Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | CD1 | Charles River | 022 | |
| Genetic reagent (Mus musculus) | C57BL/6J | JAX | JAX: 000664 | |
| Genetic reagent (Mus musculus) | Trpm7-floxed | A generous gift from Dr. Carmen P. Williams (NIEHS) (PMID: 30322909) | C57BL6/J and 129s4/SvJae mixed background | |
| Genetic reagent (Mus musculus) | Gdf9-cre | JAX | JAX: 011062 | |
| Genetic reagent (Mus musculus) | Trpv3 | A generous gift from Dr H. Xu (PMID: 20403327) | C57BL/6J and 129/SvEv mixed background | |
| Biological sample (mouse oocyte) | Mus musculus | this paper | Eggs at the metaphas e of the second meiosis | |
| Biological sample (mouse sperm) | Mus musculus | Matured sperm from cauda epididymis | ||
| Recombinant DNA reagent | pcDNA6-mouse Plcz1- venus (plasmid used as a template for mRNA synthesis) | Published in previous Fissore lab paper PMID: 34313315. Mouse Plcz1 sequence was a generous gift from Dr. Kiyoko Fukami (PMID:18028898 ) | mouse Plcz1 mRNA was fused with Venus and inserted in pcDNA6 vector | |
| Recombinant DNA reagent | pcDNA6-CALR-D1ER-KDEL (plasmid used as a template for mRNA synthesis) | Published in previous Fissore lab paper PMID: 24101727. Original D1ER vector was a generous gift from Dr. Roger Y Tsien (PMID: 15585581) | FRET construct D1ER was inserted between ER-targeting sequence of calreticuli n and kdel ER retention signal in pcDNA6 vector | |
| Recombinant DNA reagent | pcDNA6-human ERp44- HA (plasmid used as a template for mRNA synthesis) | This paper. Original human ERp44 sequence was a generous gift from Dr. Roberto Sitia (PMID: 11847130) | human ERp44 mRNA fused with HA in pcDNA6/ Myc-His B vector | |
| Antibody | Monoclonal HA (Mouse monoclonal) | Roche | 11581816001 | Dilution: 1:200 |
| Antibody | Polyclonal IP3R1 (Rabbit polyclonal) | (Parys et al., 1995) | Dilution: 1:1000 | |
| Antibody | Monoclonal α-tubulin (Mouse monoclonal) | Sigma-Aldrich | T-9026 | Dilution: 1:1000 |
| Antibody | Alexa Fluor 488 (goat anti mouse) | Invitrogen | Invitrogen: A32723 | Dilution: 1:400 |
| Commercial assay or kit | T7 mMESSAGE mMACHINE Kit | Invitrogen | Invitrogen: AM1344 | Used for in vitro mRNA synthesis |
| Commercial assay or kit | Poly(A) Tailing Kit | Invitrogen | Invitrogen: AM1350 | Used for poly (A) tailing of synthesiz ed mRNA |
| Chemical compound, drug | Hyaluronidase from bovine testes | Sigma-Aldrich | H3506 | |
| Chemical compound, drug | 3-Isobutyl-1-methylxanthine (IBMX) | Sigma-Aldrich | I5879 | |
| Chemical compound, drug | Polyvinylpyrrolidone (PVP) (average molecular weight: 360,000) | Sigma-Aldrich | PVP360 | Used for mRNA microinje ction and ICSI |
| Chemical compound, drug | N,N, N′,N′-Tetrakis (2- pyridylmethyl) ethylenediamine (TPEN) | Sigma-Aldrich | P4413 | Prepared in DMSO and kept at −20 °C until use |
| Chemical compound, drug | Zinc Pyrithione (ZnPT) | Sigma-Aldrich | PHR1401 | Prepared in DMSO and kept at −20 °C until use |
| Chemical compound, drug | Strontium chloride hexahydrate (SrCl2) | Sigma-Aldrich | 255521 | Freshly dissolved in water on the day of experime nt |
| Chemical compound, drug | Calcium chloride dihydrate (CaCl2) | Sigma-Aldrich | C3881 | Freshly dissolved in water on the day of experime nt |
| Chemical compound, drug | Magnesium chloride hexahydrate (MgCl2) | Sigma-Aldrich | M2393 | Freshly dissolved in water on the day of experime nt |
| Chemical compound, drug | Zinc sulfate monohydrate (ZnSO4) | Acros Organics | 389802500 | Freshly dissolved in water on the day of experime nt |
| Chemical compound, drug | Ethylenediaminetetraacet ic acid sodium dihydrate (EDTA) | LabChem | LC137501 | Prepared as 0.5M aqueous solution with pH 8.0 adjusted by NaOH |
| Chemical compound, drug | Diethylenetriaminepentaa cetic acid (DTPA) | Sigma-Aldrich | D6518 | |
| Chemical compound, drug | Tris (2-pyridylmethyl) amine (TPA) | Santa Cruz | sc-477037 | |
| Chemical compound, drug | Dimethyl sulphoxide (DMSO) | Sigma-Aldrich | D8418 | Used as a solvent |
| Chemical compound, drug | Acetylcholine chloride | Sigma-Aldrich | A6625 | |
| Chemical compound, drug | Thimerosal | Sigma-Aldrich | T5125 | Freshly dissolved in water on the day of experime nt and kept on ice until use |
| Chemical compound, drug | Ionomycin calcium salt | Tocris | 1704 | Working concentration: 2.5 μM |
| Chemical compound, drug | Thapsigargin | Calbiochem | #586500 | Working concentra tion: 10 μM |
| Other | Pluronic F-127 (20% solution in DMSO) (Pluronic acid) | Invitrogen | P3000MP | |
| Other | Fura-2 AM | Invitrogen | F1221 | Used at 1.25 μM in TL- HEPES containing 0.02% Pluronic acid |
| Other | FluoZin-3 AM | Invitrogen | F24195 | Used at 1.25 μM in TL- HEPES containing 0.02% Pluronic acid |
| Other | Fluo-4 AM | Invitrogen | F14201 | Used at 1.25 μM in TL- HEPES containing 0.02% Pluronic acid |
| Other | Rhod2-AM | Invitrogen | R1244 | Used at 2.2 μM in TL- HEPES containing 0.02% Pluronic acid. |
| Other | ci-IP3/ PM | Tocris | 6210 | Dissolved in DMSO and kept at −20 °C. Before use, the stock was diluted with water to make a final concentra tion of 0.25 mM. |
| Other | Pme1 | New England BioLabs | R0560S | Used to linearize pcDNA6 vectors for mRNA synthesis |
| Software, algorithm | Prism | GraphPad Software | Version 5.01 |