TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and description | Source or reference |
|---|---|---|
| E. coli | ||
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ (traD36 proAB+ lacIqlacZΔM15) | 53 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir R6K | 28 |
| V. parahaemolyticus | ||
| TH3996 | Clinical isolate, trh+ ure+ | 51 |
| TH3996(trh) | TH3996, trh disrupted | 51 |
| TH3996(nikD) | TH3996, nikD disrupted | This study |
| TH3996(ureR) | TH3996, ureR disrupted | This study |
| TH3996(ureC) | TH3996, ureC disrupted | This study |
| Plasmids | ||
| pUC119 | Cloning vector, Ampr | 40 |
| pT7-Blue | Multicopy (ColE1 ori) TA cloning vector, Ampr | Novagen, Inc. |
| pKY719 | R6K-ori suicide vector for gene replacement, Cmr | 33 |
| pKS1 | 11.8-kb XhoI fragment cloned into the SalI site on pUC119 | This study |
| pKS2 | 7.3-kb BglII fragment cloned into the BamHI site on pUC119 | This study |
| pKS3 | 4.0-kb SpeI fragment cloned into the XbaI site on pUC119 | This study |