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[Preprint]. 2023 Apr 17:2023.04.16.23288635. [Version 1] doi: 10.1101/2023.04.16.23288635

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Figure 1. — A) Temperature trends in Paraguay between 1981 and 2022. The yearly mean (red full line), yearly minimum and maximum (grey full lines), yearly 50% quantiles (dark grey area) and min, max and mean temperature in 1981 (dashed grey and red lines, respectively) are shown; B) Map of Paraguay showing the number of CHIKV genome sequences by departments. The size of the circles indicates the number of new genomes generated in this study; C) Weekly notified chikungunya cases (grey), incidence normalized per 100 K individuals (blue) and cumulative deaths (green) in 2022-2023 (until EW11), red bars at the bottom highlight the dates of sample collection of the genomes generated in this study; D) Boxplot of the patient’s (representing the study population) age and clinical outcomes value distribution. The Kruskal-Wallis non-parametric approach was used to determine the strength of association within the different clinical outcomes. E) Time-resolved maximum likelihood tree including the newly complete genome sequence from Paraguay (n=179) generated in this study combined with publicly available sequences (n=715) from GenBank collected up to March 30, 2023. Colors indicate geographic location of sampling. Support for branching structure is shown by bootstrap values at key nodes.