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[Preprint]. 2023 Apr 21:rs.3.rs-2753443. [Version 1] doi: 10.21203/rs.3.rs-2753443/v1

Figure 2. Tactile sensitivity before and after intra-articular injection of IDO-Gal3 or saline in MCLT+MMT operated knees.

Figure 2

The 50% paw withdrawal threshold in MCLT+MMT operated limbs decreased at week 5 and week 7 relative to week 0 (^, p<0.05), indicating a heightened sensitivity to touch caused by MCLT+MMT surgery. Following intra-articular injections, 50% paw withdrawal thresholds increased in both saline and IDO-Gal3 treated animals relative to pretreatment (#, p<0.05), with 6 of 7 rats returning to baseline sensitivity levels in the IDO-Gal3 group by week 9. The average tactile sensitivity for IDO-Gal3 and saline treated animals did not vary after treatment; however, when change in tactile sensitivity relative to each animal’s week 7 value was assessed (paired analysis), increases in tactile sensitivity in IDO-Gal3 treated animals were larger than saline treated animals at weeks 9–11 (p≤0.041, Fig. 2B). As Chaplan’s up-down protocol provides a discrete set of data, results are shown as box plots with median, 25–75% interquartile range, and the lower and upper fence. Data outside the fence are plotted as individual data points.