Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 1;37(7-8):303–320. doi: 10.1101/gad.350078.122

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Zacarías-Fluck et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 2. — Omomyc induces transcriptional modulation in the A375 melanoma human cell line in vitro. (A) Omomyc-RFP levels determined by cytometry, with percentage of positive cells and mean intensity. (B) Principal component analysis showing the separation of the five Omomyc-expressing (Dox) replicates from the control ones. (C) Heat map of A375 cells expressing Omomyc or not at day 4 (absolute log FC > 2.25). (D) Volcano plot of A375 cells expressing Omomyc. (E) qPCR validation of microarray results of selected genes at 6, 12, 24, and 96 h of Omomyc expression. (F) Heat maps of the top 10 regulated genes in selected gene sets. Representative MYC-related (G) and melanoma and other skin cancer (H) gene sets modulated by Omomyc. (I) Immune-related gene set enrichment induced by Omomyc expression.