Figure 1.

Neutrophil Abca1/g1 deficiency does not affect NETosis in atherosclerotic plaques of Ldlr^−/− mice. Ldlr^−/− mice were transplanted with bone marrow (BM) from Abca1^fl/flAbcg1^fl/fl (control) or S100A8CreAbca1^fl/flAbcg1^fl/fl (S100A8-Cre-Abc^dko) mice and fed Western-type diet (WTD) for 8 weeks. (A–B) Ly6G⁺ neutrophils (A) and Ly6G⁻CD11b⁺ monocytes (B) were isolated from BM and Abca1 and Abcg1 mRNA expression was assessed. n = 5. *P < 0.05, by t-test. (C) Mice were sacrificed and hearts were isolated, sectioned, and neutrophils were stained in atherosclerotic lesions of the aortic root using myeloperoxidase (MPO). Lesions were also stained for citrullinated histones 2, 8, and 17 (3HCit). NETs show an overlap of MPO and 3HCit staining. Representative pictures are shown. Scale bars: 25 μm.