Skip to main content
. 2022 Dec 20;119(4):969–981. doi: 10.1093/cvr/cvac189

Figure 2.

Figure 2

Macrophage Abca1/g1 deficiency induces neutrophil accumulation and NETosis in atherosclerotic lesions. Ldlr−/− mice were transplanted with BM from Abca1fl/flAbcg1fl/fl (control) or CX3CR1CreAbca1fl/flAbcg1fl/fl (CX3CR1Cre-Abcdko) mice and fed WTD for 8 weeks. Ly6G−CD11b+ splenic monocytes/macrophages (A) and Ly6G+ splenic neutrophils (B) were isolated and Abca1 and Abcg1 protein expression was assessed by Western blot, corrected for β-actin, and quantified. n = 4. Unedited gels are shown in Supplementary material online, Figure S16. (C) In atherosclerotic lesions, neutrophils were stained using MPO, and the MPO+ percentage of lesion size was quantified. Lesions were also stained for 3HCit. To assess NETs, the overlap of MPO and 3HCit was quantified. Representative pictures are shown. Scale bars: 75 μm. Each datapoint represents an individual mouse. n = 12. ***P < 0.001, by t-test.