Figure 3.

Macrophage Abca1/g1 deficiency induces inflammasome activation in monocytes, macrophages, and neutrophils of Ldlr^−/− mice. Ldlr^−/− mice were transplanted with BM from Abca1^fl/flAbcg1^fl/fl (control) or CX3CR1CreAbca1^fl/flAbcg1^fl/fl (CX3CR1Cre-Abc^dko) mice and fed WTD for 8 weeks. Ly6G⁻CD11b⁺ splenic monocytes/macrophages (A) and Ly6G⁺ splenic neutrophils (B) were isolated and caspase-1 cleavage was assessed by Western blot. To quantify caspase-1 cleavage, the p20 cleaved form of caspase-1 was divided by the p45 pro-form. n = 4–6. (C) Plasma IL-18 levels. (D–E) IL-1β secretion from control or CX3CR1Cre-Abc^dko BMDMs treated with 20 ng/mL LPS for 3 h, and with 10 µg/mL nigericin for an additional 1 h. (D) IL-1β secretion was assessed by ELISA. (E) Immunoblot of IL-1β cleavage of cell lysates. Unedited gels are shown in Supplementary material online, Figure S17. Each datapoint represents an individual mouse. n = 14. *P < 0.05, ** P < 0.01, ***P < 0.001 (A–C) by t-test and (D–E) by one-way ANOVA.