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. Author manuscript; available in PMC: 2023 May 3.
Published in final edited form as: ACS Biomater Sci Eng. 2020 May 27;6(6):3454–3463. doi: 10.1021/acsbiomaterials.9b01792

Figure 4.

Figure 4.

μRB scaffolds promote robust and interconnected collagen regardless of stiffness. (A) Gross morphology of μRB scaffolds with varying stiffness and with or without MSCs on days 1 and 31. Increase in sample opacity suggests matrix deposition. The soft formulation was unable to maintain its physical integrity. Scale bar, 2 mm (B) Masson’s Trichrome staining for total collagen on day 31. (C) ARS staining for bone mineralization. Low-magnification (upper row) and high-magnification (lower row) images are provided for both stains. Scale bars in low-magnification images, 2 mm; scale bars in high-magnification images, 200 μm.