Extended Data Fig. 4. Depletion of TNF-α from SARS-CoV-2-infected cells by siRNA reduces senescence-inducing activity.
a, Early passage HDFs were cultured for 9 days with culture supernatant of ACE2-HDFs infected with SARS-CoV-2 for 6 days in the presence of indicated siRNA. b, RT-qPCR analysis of TNFα and genomic RNA of SARS-CoV-2 in ACE2-HDFs infected with SARS-CoV-2 with or without siRNA-mediated depletion of indicated genes. c, Representative photographs (upper panel) and RT-qPCR analysis (lower panel) of HDFs incubated with culture supernatant of SARS-CoV-2-infected ACE2-HDFs for 9 days (upper panels). For all graphs, error bars indicate mean ± standard deviation (s.d.) of biological triplicate. Statistical significance was determined with one-way ANOVA followed by Dunnett’s multiple comparison test in (b) and (c).