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. 2023 Mar 16;24(5):827–840. doi: 10.1038/s41590-023-01468-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Flow cytometry gating strategy to show Lineage(Lin)⁻Sca-1⁺c-Kit⁺ (LSK) multipotent progenitors, Lin⁻Sca-1⁻c-Kit^int/hi myeloid lineage-committed progenitors (MyP) and Lin⁻Sca-1^intc-Kit^int common lymphoid progenitors (CLP). b-c, Representative histograms of YFP expression (b) and bar graphs showing % of YFP⁺ cells (c) in BM LSK, MyP and CLP (c) from Tmem119^+/+Rosa26^LSL-EYFP (blue) and Tmem119^Cre/+Rosa26 ^LSL-EYFP (red) mice. d-e, Representative histograms of YFP expression (d) and bar graphs showing % of YFP⁺ cells (e) in blood CD45⁺CD3⁺CD19⁻ T cells, CD45⁺CD3⁻CD19⁺ B cells, CD45⁺CD3⁻CD19⁻Ly6G⁻SiglecF⁻CD115⁺ Ly6C⁺ cMo or Ly6C⁻ pMo, CD45⁺CD3⁻CD19⁻Ly6G⁺CD11b⁺ neutrophils (Neu), CD45⁺CD3⁻CD19⁻CD11b⁺SiglecF⁺ eosinophils (Eos) from Tmem119^+/+Rosa26^LSL-EYFP (blue) and Tmem119^Cre/+Rosa26 ^LSL-EYFP (red) mice. f-g, Representative histograms of YFP expression (f) and bar graphs showing % of YFP⁺ cells (g) in lung cMo, pMo, cDC1, cDC2, AMs, IMs and CD45⁻ structural cells from Tmem119^+/+Rosa26^LSL-EYFP (blue) and Tmem119^Cre/+Rosa26 ^LSL-EYFP (red) mice. h-i, Representative histograms of YFP expression (h) and bar graphs showing % of YFP⁺ cells (i) in small and large peritoneal macrophages (SPM and LPM, respectively), Kupffer cells (KC), red pulp splenic macrophages (RPM), small intestinal (SI) and colon (C) lamina propria macrophages (LPM) and microglia from Tmem119^+/+Rosa26^LSL-EYFP (blue) and Tmem119^Cre/+Rosa26 ^LSL-EYFP (red) mice. j, Numbers of microglia quantified by flow cytometry in IM^DTR and littermate control mice injected i.p. with 50 or 500 ng DT and evaluated 24 or 72 h post-DT injection. Data show mean ± SEM and are pooled from 2 independent experiments (c,e,g,i,j) (c,e,g,i: Tmem119^+/+Rosa26^LSL-EYFP; Tmem119^Cre/+Rosa26 ^LSL-EYFP: n = 4;6 mice per group, respectively; j: n = 3-4 mice per group). P values were calculated using a two-way (c,e,g,i) or a one-way (j) analysis of variance (ANOVA) with Tukey’s post hoc test. In g, P values compare IMs vs. every other population. Raw data and P values are provided as a source data file. ^*, P < 0.05; ^****, P < 0.0001. ns, not significant.

Source data