Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 16;24(5):827–840. doi: 10.1038/s41590-023-01468-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, Representative plots of Ly6C and CD64 expression within lung AM-excluded CD45⁺F4/80⁺SSC^loCD11b⁺ cells at days 0, 0.5, 1, 2, 3, 7 and 14 after 50 ng DT i.p. in IM^DTR mice. b, Time course of absolute numbers of cMo, pMo, AMs, bulk IMs, CD206⁻ IMs and CD206⁺ IMs quantified by flow cytometry in IM^DTR and littermate controls, as in a. Data show the mean (centerline) ± s.e.m. (colored area) and are pooled from ≥2 independent experiments (n = 8–10 mice per time point). c, Amount of Ccl2 in the lung and serum of IM^DTR and littermate controls at 0, 12, 24 and 48 h after DT i.p. injection. d,e, Representative CD45.1 and CD45.2 contour plots (d) and bar graphs showing the percentage of CD45.1 donor and CD45.2 host chimerism (e) in the indicated cell populations from lethally irradiated thorax-protected CD45.2 IM^DTR mice reconstituted with CD45.1 wild-type BM donor cells, injected or not with 50 ng DT i.p. 4 weeks later and evaluated at day 7 after DT. f,g, Representative CD45.1 and CD45.2 contour plots (f) and bar graphs showing the percentage of Ccr2^+/+ donor, Ccr2^−/− donor and host chimerism (g) in the indicated cell populations from lethally irradiated, thorax-protected CD45.1/CD45.2 IM^DTR mice transplanted with a 1:1 mix of CD45.2 Ccr2^−/− and CD45.1 Ccr2^+/+ BM cells, injected with 50 ng DT i.p. 4 weeks later and evaluated at day 7 after DT. h, Representative contour plot of Ly6C and CD64 expression within lung single live AM-excluded CD45⁺F4/80⁺SSC^loCD11b⁺ cells in CD45.1/CD45.2 IM^DTR mice treated with 50 ng DT i.p., transferred with CD45.1 BM wild-type cMo i.v. 24 h after DT and evaluated at days 2 and 14 after DT. Plots are representative of 5 mice, each of them giving similar results. Data show the mean ± s.e.m. and are pooled from two independent experiments (c, e and g: n = 4–8, 4–8 and 6 mice per group, respectively). P values were calculated by likelihood ratio tests (b), two-way ANOVA with Tukey’s post hoc tests (c,e) or one-way ANOVA with Sidak’s post hoc tests (g). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Source data