Fig. 6. Microgliosis and astrogliosis are significantly reduced after neuronal APOE4 removal.
a, Representative images of microglia immunostaining with anti-Iba1 in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre (scale bar, 500 µm). b, Quantification of the percent Iba1 coverage area in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre. c, Correlation between percent Iba1 coverage area and hippocampal volume of PS19-fE4 mice (n = 25). d, Representative images of activated microglia immunostaining with anti-CD68 in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre (scale bar, 500 µm). e, Quantification of percent CD68 coverage area in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre. f, Correlation between percent CD68 coverage area and hippocampal volume of PS19-fE4 mice (n = 25). g, Representative images of astrocyte immunostaining with anti-GFAP in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre (scale bar, 500 µm). h, Quantification of percent GFAP coverage area in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre. i, Correlation between percent GFAP coverage area and hippocampal volume of PS19-fE4 mice (n = 25). j, Representative images of activated astrocyte immunostaining with anti-S100β in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre (scale bar, 500 µm). k, Quantification of percent S100β coverage area in the hippocampus of 10-month-old PS19-fE4 and PS19-fE3 mice with and without Cre. l, Correlation between percent S100β coverage area and hippocampal volume of PS19-fE4 mice (n = 25). For quantifications in b,e,h,k, PS19-fE4: No Cre, n = 25; Syn1-Cre, n = 17; and PS19-fE3: No Cre, n = 20; Syn1-Cre, n = 15 mice. All data are represented as mean ± s.e.m., one-way ANOVA with Tukey’s post hoc multiple comparisons test. Pearson’s correlation analysis (two-sided). Source data.