Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 20;2(9):837–850. doi: 10.1038/s43587-022-00281-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, Experimental design of hippocampal PSD proteome analysis. Hippocampi from 6- and 9-month-old male mice were dissected and isolated synapse fractions were analyzed by TMT multiplex proteomics (Methods). b, Venn diagram showing the number of identified proteins and overlap between synapse proteomes in the 6- and 9-month cohort from this study and synapse proteome from 9-month-old female mice described previously³. c, Percentage of DE proteins in the indicated genotype comparisons at 6 and 9 months. d, A heat map showing z scores across genotypes for proteins that were DE between C1qKO and WT mice (regardless of P301S genotype) (P ≤ 0.05; FC ≥ 5). e,f, Volcano plots showing the comparison between P301S versus WT and P301S;C1q^KO versus WT synapse proteomes at 6 and 9 months. MSstats was used to calculate log₂FC and standard error utilizing a linear mixed-effects model that considered quantification from each peptide and biological replicate per protein. P values were then calculated by comparing the model-based test statistic to a two-sided Student’s t-test distribution. Significantly up- and downregulated proteins (P < 0.05, log₂FC ± 0.5) are shown in blue and red circles, respectively. Selected DE proteins are labeled with their protein or gene name. g, Selected up- or downregulated KEGG pathways in P301S versus WT and P301S,C1q^KO versus WT synapse proteomes at 6 and 9 months. Only DE proteins were included for pathway analysis.

Source data