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. 2022 Aug 15;2(8):742–755. doi: 10.1038/s43587-022-00263-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a Schematic of Ionizing radiation (IR)-induced senescence. b Representative growth curve of cells undergoing replicative senescence (RS). c Representative micrographs of SA-β-gal activity and DAPI in control, IR and RS cells; scale bars, 10 μm. d Relative p21 mRNA expression levels by qPCR (n = 3, ± SEM). e Relative p16 mRNA expression by qPCR (n = 3, ± SEM). f Relative IL-6 mRNA expression by qPCR (n = 3, ± SEM). g Representative immunohistochemistry micrographs of nuclei with p21, p16, and p53 staining in control and IR cells; scale bars, 10 μm. h Distribution of p16 intensities for IR and control for three fibroblast cell lines (n = 9,196-27,716). i Distribution of p21 intensities for IR and control for three fibroblast cell lines (n = 13,678-32,730). j Distribution of p53 intensities for IR and control for three fibroblast cell lines (n = 12,844-31,100). k Cell count following irradiation (n = 3 cell lines, mean ± 95% CI). l DAPI intensities for IR, RS, and control for three fibroblast cell lines (RS n = 6,976, IR n = 19,193, control n = 68,971; mean ± 95% CI, two-sided Student’s t-test). m Gating strategy for cell cycle analysis (data shown is for 10 Gy treated fibroblasts), mn: multinucleated cells