Fig. 1. Lifespan response to rapamycin treatment declines with the age of onset of the treatment.
a, Rapamycin treatment started on day 30 extended lifespan (P = 2.13 × 10−6) to a lesser degree than did lifelong treatment (P = 1.04 × 10−13; Supplementary Table 2). b,c, Rapamycin treatment started on day 45 modestly extended lifespan (b; P = 0.0003; Supplementary Table 1), whereas treatment started on day 60 (c) had no lifespan-extending effect (P = 0.256; Supplementary Table 1). d, Rapamycin treatment from day 1–30 extended lifespan (P = 2.13 × 10−6) as much as did chronic treatment (P = 0.09; Supplementary Table 2). e, Treatment from days 15–30 extended lifespan slightly less than did chronic treatment (d15–30 versus control, P = 7.58 × 10−7; d15–30 versus chronic rapamycin, P = 0.19; Supplementary Table 3). Experiments in Extended Data Fig. 1a–d and Figs. 1e and 2a were run in parallel, thus lifespan data of the control flies are the same. N = 400 flies per condition.