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. 2022 Aug 29;2(9):824–836. doi: 10.1038/s43587-022-00278-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Number of punctae stained by LysoTracker and Cyto-ID in 25-day-old flies treated with rapamycin either chronically or in days 1–15 (n = 7). b,c, Immunoblot of autophagy-related proteins, Atg8-I, Atg8-II and Ref-2-P in the fly gut (b) and the head (c) on day 25, 10 d after rapamycin treatment. Data are the mean ± s.e.m. One-way ANOVA; Bonferroni’s multiple-comparison test. n = 4 biological replicates, each consisting of ten flies.

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