Extended Data Fig. 9. Elevated brain soluble ST2 level exacerbates amyloid-beta deposition in female but not male 5XFAD mice.

(a, b) Amyloid-beta (Aβ) deposition in the cortices of 4-month-old male and female 5XFAD mice after 28-day intracerebroventricular delivery of soluble sST2 (sST2)-Fc or Fc as a control. (a) Representative images of Aβ staining. Scale bar, 1 mm. (b) Quantification of Aβ plaques (% of total cortical area) (control: n = 6 male and 5 female mice, sST2: n = 6 male and 5 female mice). T = 0.590 (males) and 2.721 (females); P = 0.568 (males) and 0.026 (females). Data in bar charts are mean + SEM. Statistical tests were performed as two-sided unpaired Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001.