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. 2023 Mar 9;3(3):327–345. doi: 10.1038/s43587-023-00373-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Profiling of animals and their derived brain cells used for sequencing, before (N = 56) (a,c,e) and after (n = 50) (b,d,f) quality control filtering in which certain animals were omitted (see Methods). Boxplot minimum is the smallest value within 1.5 times the interquartile range below the 25^th percentile, maximum is the largest value within 1.5 times the interquartile range above the 75^th percentile. Boxplot center is the 50^th percentile (median), box bounds are the 25^th and 75^th percentile. Outliers are >1.5 times and < 3 times the interquartile range. a-b. Age of mice in weeks prior to parabiosis surgeries. c-d. Number of days joined across parabiotic pairs. e-f. Number of dissociated cells analyzed per brain across all animal types.