Extended Data Fig. 5. C5-T_SE OT1 TILs are polyfunctional effector cells.

a Analysis of KLRG1, CX₃CR1 and LY6C expression in CD44⁺ PD-1⁺ TCF1^neg GZMC⁺ OT1 TILs at day 12 post orthogonal ACT. b Representative dot plots showing the expression of effector molecules in CD44⁺PD-1⁺TCF1^negGZMC⁺ OT1 TILs harvested at day 12 post orthogonal ACT and 4 h in vitro stimulated with 10 ng/mL of cognate SIINFEKL peptide in the presence of Brefeldin A. GO: Biological Process (c) and Reactome pathway (d) over-representation tests of genes upregulated in T_EX from the reference TIL map²¹, relative to C5-T_SE TILs. Reactome-Pathway and GO enrichment were performed with the functions, enrichGO and enrichReatome, respectively, from the same package, which use a one-sided version of the Fisher’s exact overrepresentation test to find enriched categories. e Top: Schematics showing the experimental protocol to in vitro generate bona-fide exhausted OT1 T cells. Bottom: Analysis of the expression of PD-1, TOX, TCF1, IFNγ and TNFα in multiple stimulated OT1 cells (red). OT1 cells that received one single stimulation (blue) or none (black), were used as controls.