Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 24;30(5):1155–1165. doi: 10.1038/s41418-023-01126-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Schematic representation of the treatment of murine organoids seeded as single cells with recombinant IL-6 or LIF and timing of quantification of the morphology and gene expression signatures. B Representative brightfield images of YFP + EpCAM + (E+; N = 6), YFP + EpCAM- (E-) and YFP + secondary (Met; N = 12) tumor organoids on day 3 post treatment (POST) with the indicated cytokine. Organoids are grouped as glandular (G) and solid (S) morphologies pre-treatment (PRE). Scale bar = 300 μm. C Quantification of the tumor organoid morphology 3 days post treatment with the indicated cytokine. YFP + EpCAM + (E + , N = 6), YFP + EpCAM- (E-, N = 12 primary and secondary). Glandular (green), solid (red) and branching (blue). Data is presented + /− SEM. Results are not significant, Chi-square test. D mRNA expression levels of S100 family proteins, S100a4 and S100a14, in YFP + EpCAM + (E+), YFP + EpCAM- (E-) primary tumor organoids and YFP + secondary tumor organoids following the addition of the indicated cytokine (IL-6, brown; LIF, red). Each dot represents an individual organoid. Organoids are grouped as glandular (G) and solid (S) morphologies. Data includes 3 biological replicates and is presented as log10 fold change relative to the vehicle (white) control, mean + /− SEM. paired t-test.