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. 2023 Mar 3;30(5):1334–1348. doi: 10.1038/s41418-023-01142-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a QRT-PCR analysis of HOXC13-AS expression in keratinocytes treated with wound-related cytokines and growth factors for 24 h (n = 4). b QRT-PCR analysis of total and nascent HOXC13-AS in keratinocytes treated with EGF for 8 h (n = 3). c QRT-PCR analysis of HOXC13-AS in keratinocytes treated with EGF and then actinomycin D for 0–24 h (n = 4). d QRT-PCR analysis of HOXC13-AS in keratinocytes treated with AG-1478 for 24 h (n = 4). Expression correlation of HOXC13-AS with HBEGF in the skin and wound tissues (e) and the isolated cells (f) analyzed by RNA-seq. g QRT-PCR analysis of HOXC13-AS in keratinocyte progenitor cells and calcium-induced differentiated keratinocytes (n = 4). Representative photographs (h) and quantification (i) of HOXC13-AS FISH in keratinocyte progenitor cells and differentiated keratinocytes treated with 1.5 mM calcium for 3 days (n = 3). Cell nuclei were co-stained with DAPI. Scale bar = 20 μm. ns not significant, *p < 0.05; **p < 0.01, ***p < 0.001 and ****p < 0.0001 by unpaired two-tailed Student’s t test (a, b, d, g, i), two-way ANOVA (c), or Pearson’s correlation test (e and f). Data are presented as mean ± SD.