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. 2023 Mar 13;30(5):1184–1197. doi: 10.1038/s41418-023-01147-8

Fig. 2. DCLK1 mediates inflammatory responses through NF-κB.

Fig. 2

A RAW264.7 cells were transfected with shDCLK1. Control cells were transfected with negative control shRNA (scrambled; shNC). Knockdown efficiency was determined [n = 3; Mean ± SEM]. B RAW264.7 transfected with DCLK1 shRNA were exposed to 0.5 μg/mL LPS for 6 hours. RNA was sequenced to identify differentially expressed genes. Figure showing a Venn diagram of genes upregulated in LPS compared to control (red), and genes downregulated in shDCLK1 + LPS compared to LPS (blue). C Pathway analysis of genes that are increased by LPS but restored in shDCLK1 + LPS. D Transcription factor prediction was performed using PASTAA, chEA2016, ChIP 2015, and TRRUST 2019. E Heatmap showing select NF-κB-target genes which were increased by LPS and restored in shDCLK1 + LPS. F qPCR validation of NF-κB-regulated genes in LPS-challenged RAW 264.7 cells, with or without DCLK1 shRNA transfection. Cells were treated as indicated in B [n = 3; Mean ± SEM; *p < 0.05 and ***p < 0.001].