Fig. 3.

A Golgi–Cox staining showed that Tarooneh extract increases neuronal spine density in the pyramidal CA1 neurons after the chronic restraint stress. B Exemplary photomicrographs of stained neurons in the hippocampal CA1 neurons. (a) Control; (b) Stress; (c) Tarooneh; (d) Stress + Tarooneh groups. Three animals in each groups were used for Golgi–Cox staining. Then the images were analyzed with the ImageJ software. The number of spine density on each neuron per 100 μm was estimated. For each group, three animals were selected, five slides were analyzed, and, of all sections, a minimum of seven neurons was selected on each rat. The scale bar represents 100 μm. All data are presented as mean ± SEM. **P = 0.0008, *as a P < 0.0052