Figure 1.

Schematic diagram illustrating the two methods of VEGF administration to axotomized abducens motoneurons.

(A) One approach consisted of the administration of VEGF through the transected sixth nerve inserted into an intraorbital device (IOD). (B) In a different experimental procedure, VEGF was administered in the fourth ventricle with a micropipette through the intact cerebellum (CB). Abducens motoneurons were electrophysiological recorded (Rec.) following their antidromic identification carried out after electrical stimulation (St.) to the nerve. Eye coils were used for the recording of eye movements. LR: Lateral rectus muscle, the extraocular muscle innervated by abducens motoneurons; VEGF: vascular endothelial growth factor.