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. 2022 Nov 9;18(8):1818–1826. doi: 10.4103/1673-5374.360242

Figure 3.

Figure 3

Effects of exendin-4 and linagliptin on dopaminergic neurons in the SN and cell terminals in the STR.

(A, B) Immunohistochemical staining for TH in the SNpc and STR. The MPTP group showed weaker staining for TH than the CON group, while the MPTP + EX-4 and MPTP + LINA groups showed stronger staining for TH than the MPTP group. Scale bar: 200 µm, 40 µm for low and high magnification, respectively. (C–E) Western blot assay for TH and DAT expression in SN tissue. (F) Quantitation of TH-positive cells (/100-fold magnification) in the SNpc as detected by immunohistochemistry. Data are presented as mean ± SD (n = 3). *P < 0.05, ***P < 0.001, ****P < 0.0001, vs. CON; #P < 0.05, ##P < 0.01, vs. MPTP (one-way analysis of variance followed by Tukey’s post hoc test). CON: Control; DAT: dopamine transporter; EX-4: exendin-4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; LINA: linagliptin; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; SNpc: substantia nigra pars compacta; STR: striatum; TH: tyrosine hydroxylase.