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. 2022 Nov 25;18(8):1841–1846. doi: 10.4103/1673-5374.361533

Figure 5.

Figure 5

Exercise and ADSCs ameliorate mechanical sensitivity after SCI.

(A, B) von Frey filament testing with 0.07 g (A) and 0.16 g forces (B) was performed to detect mechanical allodynia. Animals in the SCI, SCI + exercise, SCI + ADSCs, and SCI + exercise + ADSCs groups developed mechanical allodynia after 7 days post-injury (dpi). However, animals in the SCI + ADSCs and SCI + exercise + ADSCs groups showed a lower withdrawal response rate compared with those in the SCI and SCI + exercise groups (P < 0.05, P < 0.01, or P < 0.001). Both exercise and ADSCs administration significantly ameliorated SCI-induced mechanical allodynia from 14 to 28 dpi (P < 0.0001, P < 0.001, or P < 0.0001). (C, D) Mechanical hypoalgesia was evaluated via using von Frey filaments with 1 (C) and 1.4 g forces (D). At 7 dpi, animals in the SCI, SCI + exercise, SCI + ADSCs, and SCI + exercise + ADSCs groups showed lower withdrawal response rates compared with the sham group, indicating mechanical hypoalgesia (P < 0.0001). Exercise had no influence on mechanical hypoalgesia, while ADSCs administration significantly improved SCI-induced mechanical hypoalgesia (P < 0.0001). The results are presented as the mean ± SEM. ****P < 0.0001, SCI group vs. Sham group; &P < 0.05, SCI + ADSCs group vs. SCI + exercise group; ##P < 0.01, ###P < 0.001, ####P< 0.0001, SCI + exercise + ADSCs group vs. SCI + exercise group; ^^^P < 0.001 and ^^^^P < 0.0001, SCI + exercise + ADSCs group vs. SCI group (two-way analysis of variance with Fisher’s least significant difference post hoc test). ADSCs: Adipose-derived stem cells; dpi: day post-injury; SCI: spinal cord injury.