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. 2023 May 3;42(5):112503. doi: 10.1016/j.celrep.2023.112503

Figure 1.

Figure 1

ELISA cross-reactivity of anti-S monoclonal antibodies (mAbs)

(A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity.25 Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb.

(B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%.

See also Figure S1.