REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
XG anti-SARS-CoV-2 bNAbs	Zhou et al.25	N/A
XG005a	This paper	N/A
XG005b	This paper	N/A
XG005c	This paper	N/A
Ly-CoV1404	Westendorf et al.24	N/A
Goat anti-Human IgG (H + L) secondary antibody, HRP	Thermo Fisher Scientific	Cat#31410; RRID: AB_228269
Bacterial and virus strains
E. Coli trans5α chemically Competent Cells	TransGen Biotech	Cat#CD201-01
Authentic BA.2 and BA.5 virus	Guangzhou Custom Technology Center	N/A
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 Spike protein (ECD, His & FLAG tag)	GenScript	Cat#Z03481
SARS-CoV-2 variant proteins (ECD)	Wang et al.39	N/A
SARS-CoV-2 Omicron S trimer	This paper	N/A
PBS (10×), pH 7.2–7.4	Solarbio Life Sciences	Cat#P1022
1 M Tris-HCl, pH 9.0	Solarbio Life Sciences	Cat#T1160
Dimethyl Sulfoxide	Sigma	Cat#2650
Bovine Serum Albumin	WeiAo Biotech, Shanghai	Cat#WH3044
Fetal Bovine Serum	GEMINI	Cat#900-108
OPM-293 CD05	OPM biosciences	Cat# 81075-001
OPM-293 PreFeed	OPM biosciences	Cat#F081919-001
DPBS	VivaCell	Cat#C3590-0500
Opti-MEM	Gibco	Cat#31985070
SurePAGE™	Genscript	Cat#M00654
Tris-MOPS-SDS	Genscript	Cat#M00138
5× Protein loading buffer	Genscript	Cat#MB01015
Protein Marker	Genscript	Cat#M00624
InstaBlue Protein Stain Solution	APExBIO	Cat#B8226
ABTS Chromogen/substrate solution for ELISA	Thermo Fisher Scientific	Cat#00-2024
EZ trans	Life iLab Biotech Co., Ltd, Shanghai, China	Cat#AC04L082
Critical commercial assays
Superose 6 Increase 10/300 column	GE Healthcare	Cat#CA10192-228
Histrap HP	GE Healthcare	Cat#17-5248-02
Protein G Sepharose 4 Fast Flow	GE Healthcare	Cat#17061805
Pierce™ IgG Elution buffer	Thermo Scientific	Cat#21004
AgeI-HF	New England BioLabs	Cat#R3552L
BsiwI-HF	New England BioLabs	Cat#R3553L
BamH1	New England BioLabs	Cat# R0136M
XhoI	New England BioLabs	Cat#R0146L
SalI-HF	New England BioLabs	Cat#R3138S
10× NEB buffer 2	New England BioLabs	Cat#B7002S
Phusion High-Fidelity DNA Polymerase	Thermo Scientific	Cat#M0530L
T4 DNA polymerase	New England BioLabs	Cat#M0203L
T4 DNA ligase	New England BioLabs	Cat#M0202M
NucleoSpin Gel and PCR Clean-up	New England BioLabs	Cat#740690.250
Luciferase Cell Culture Lysis 5× Reagent	Promega	Cat#E1531
Firefly Luciferase Assay Kit	Promega	Cat#E1501
VigoFect	Vigorous Biotechnology	Cat#T001
HiPure Plasmid EF Maxi Kit	Magen	Cat#P1156
Deposited data
SARS-CoV-2 wild-type S trimer-XG005 fab complex structure	Liu et al.27	PDB: 7V26
SARS-CoV-2 Omicron S trimer-XG005 fab complex structure	This paper	PDB: 7YD0, 7YCY, 7YCZ, 7YD1
Experimental models: Cell lines
HEK293F cell line	Zhou et al.25	N/A
HEK293T cell line	Xia et al.40	N/A
Huh-7 cell line	Xia et al.40	N/A
Raji cell line	Zhou et al.25	N/A
Vero-E6 cell line	Guangzhou Custom Technology Center	N/A
Experimental models: Organisms/strains
Human ACE2 transgenic mice	GuangDong GemPharmatech Co., Ltd.	N/A
C57BL/6JSmoc hFcRn transgenic mice	Shanghai Model Organisms Center, China	N/A
Recombinant DNA
pNL4-3.luc.RE	Xia et al.40	N/A
pcDNA3.1-SARS-CoV-1-S	Xia et al.40	N/A
pcDNA3.1-SARS-COV-2-S	Xia et al.40	N/A
pcDNA3.1-SARS-CoV-2-S mutants	This paper	N/A
Software and algorithms
PRISM	GraphPad	https://www.graphpad.com
IgBlast	Ye et al.41	http://www.ncbi.nlm.nih.gov/igblast/
IMGT/V-QUEST	Brochet et al.42	http://www.imgt.org/IMGT_vquest/vquest
FMSB	R project	http://www.r-project.org
Swiss-model	Waterhouse et al.32	https://swissmodel.expasy.org
UCSF Chimera	Pettersen et al.43	https://www.cgl.uscf.edu/chimera/
DeepEMhancer	Sanchez-Garcia et al.44	https://github.com/rsanchezgarc/deepEMhancer
Others
Sterile 50 mL Disposable Vacuum Filtration System	Millipore Sigma	Cat#SCGP00525
Amicon Ultra-4 Centrifugal Filters Ultracel-30K	Merck Millipore Ltd.	Cat#UFC803096
Ultrafree-MC Centrifugal filter units, 0.22uM GV DURAPORE	Merck Millipore Ltd.	Cat#UFC30GV0S
General Long-Term Storage Cryogenic Tubes	Nalgene	Cat#5000-1020
SimpliAmp™ Thermal Cycler	Thermo Fisher Scientific	Cat#A24812
500 mL Bottle Top Vacuum Filter, 0.20 μm Pore	Thermo Fisher Scientific	Cat#566-0020
ACCUSPIN Tubes Sterile, 50mL Capacity	Sigma	Cat#A2055-10EA