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. 2023 Apr 19;14:1102094. doi: 10.3389/fimmu.2023.1102094

Figure 9.

Figure 9

Interference of EIF2AK3 inhibits viability and mobility of glioma cells. (A) RT-qPCR presented the relative expression of EIF2AK3 in HEB, SHG44, and HS683 cell lines. (B) RT-qPCR analysis was conducted to evaluate the interference efficiency of EIF2AK3-specific short interfering RNAs (siRNAs) in SHG44 and HS638 cells; EIF2AK3-si-1 was the most effective interfering sequence and thus applied for subsequent functional experiments. (C, D) The effects of EIF2AK3 knockdown on the proliferation of SHG44 and HS683 cells were investigated via CCK8 assays. (E) Scratch experiments revealed that EIF2AK3 knockdown inhibited migration ability in SHG44 and HS683 cell lines. *P < 0.05; **P < 0.01; ***P < 0.001.