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. 2023 Apr 19;14:1040493. doi: 10.3389/fimmu.2023.1040493

Figure 2.

Figure 2

The analysis of cytokine response in the lungs of mice with intratracheal applications of 20 µg of Alt extract and PBS-treated controls. The levels of IL-33 (A) in the lung homogenates were measured by Luminex. Full-length IL-33 (IL-33FL) and cleaved forms (IL-33C) were analysed by western blotting and one representative blot is shown (B) and area under the peak (AUP) was quantified using ImageJ software (C). Representative images of IL-33 immunostaining in the lungs of C57BL/6 and BALB/c treated with PBS or the Alt extract for six times every 48 hours (D). IL1rl1 gene expression levels analysed in lungs by RT-qPCR (E). The levels of IL-5 (F), IL-13 (G) in the lung homogenates were measured by Luminex. The data are presented as scatter plot with a bar ± standard deviation (S.D). Statistical significance between the groups was determined by one-way ANOVA with Dunn’s test for multiple comparisons. N=4-6 per group. *p < 0.05, **p < 0.01, ***p < 0.001.