Figure 5.

IL-33 degrading abilities of supernatants from C57BL/6 and BALB/c BMMCs. Schematic representation of the GFP-murine IL-33-mCherry fusion protein used for IL-33 cleavage assay with BMMCs supernatants (A). Supernatants from C57BL/6 or BALB/c BMMCs, which were either stimulated with IgE+DNP or left untreated were incubated with GFP-murine IL-33-mCherry for 1 h (B). IL-33 degradation was analysed by western blotting. Supernatants from IgE+DNP stimulated C57BL/6 or BALB/c BMMCs were incubated with GFP-murine IL-33-mCherry for 10, 30, 60, or 240 min at 37°C. IL-33 incubated only in buffer for 240 min at 37°C served as negative control. IL-33 degradation was analysed by western blotting (C). Beta-hexosaminidase degranulation assay of untreated or IgE+DNP stimulated BMMCs from C57BL/6J or BALB/c mice (D). Data are presented as mean ± SEM.