Table 1. Physiochemical and kinetic parameters of functionally characterised GH29 α-l-fucosidases.
| Taxonomy | Source | Organism | GenBank ID | SP | Subfamily | Opt pH | OptT/°C | Km (μM) | kcat (s−1) | kcat/ Km (s−1 μM−1) | Refs |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Archaea | Hot springs | Sulfolobus solfataricus P2 | Ssα-fuc/AAK43160.1 AAK43159.1 | N | A | 3.3–6.3 | 95 | 28 ± 4 | 287 ± 11 | 10.25 | [111] |
| FCB Group | Gut | Bacteroides thetaiotaomicron VPI-5482 | BT1625/AAO76732.1 | Y | B | – | – | 3200 ± 700 | 0.37 ± 0.03 | (113 ± 3) × 10−6 | [53] |
| FCB Group | Gut | Bacteroides thetaiotaomicron VPI-5482 | BT2970/AAO78076.1 | Y | A | 6 | 37 | 2600 ± 500 | 0.53 ± 0.03 | (2 ± 0.5) × 10−4 | [47,53] |
| FCB Group | Gut | Bacteroides thetaiotaomicron VPI-5482 | BT4136/AAO79241.1 | Y | B | – | – | 4500 ± 400 | 0.45 ± 0.03 | (98 ± 12) × 10−6 | [53] |
| FCB Group | Warm spring | Emticicia oligotrophica | Eo0918/AFK04462.1 | N | B3 | 6–7 | 30–45 | 750 ± 110 | 3.73 ± 0.31 | 4.9 × 10−3 2 | [113] |
| FCB Group | Warm spring | Emticicia oligotrophica | Eo3066/AFK02389.1 | N | A3 | 6–7 | 30–45 | 8630 ± 1730 | 0.04 ± 0.01 | 4.6 × 10−6 2 | [113] |
| FCB Group | Warm spring | Emticicia oligotrophica | Eo3812/AFK05193.1 | N | A3 | 6–7 | 30–45 | 8410 ± 2060 | 3.61 ± 0.23 | 4.3 × 10−4 2 | [113] |
| FCB Group | Oral | Tannerella forsythia ATCC 43037 | TfFuc1/AEW21393.1 | N | A3 | 9 | – | 670 ± 200 | 17.27 ± 0.681 | 0.026 2 | [114] |
| FCB Group | Lymphoma patient | Elizabethkingia meningoseptica FMS-007 | cFase I/WP_047034007.1 | Y | A3 | 4.5 | 55 | 600 ± 50 | 0.14 ± 0.003 | (232 ± 6.7) × 10−6 | [55] |
| FCB Group | Marine | Wenyingzhuangia fucanilytica CZ1127T | Alf1_Wf/ANW96380.1 | Y | A | 7 | 30 | 3300 ± 420 | 5.44 | 1.65 × 10−3 | [32] |
| FCB Group | Marine | Wenyingzhuangia fucanilytica CZ1127T | FucWf1/ANW96121.1 | Y | A | 6.3 | 25 | 500 | 19.94 ± 4.131 | 0.040 2 | [46] |
| FCB Group | Marine | Wenyingzhuangia fucanilytica CZ1127T | FucWf2/ANW96113.1 | Y | A | 6.3 | 25 | 670 | 5.88 ± 0.821 | 8.8 × 10−3 2 | [46] |
| FCB Group | Marine | Wenyingzhuangia fucanilytica CZ1127T | FucWf3/ANW96108.1 | Y | A | 6.3 | 30 | 2210 | 0.20 ± 0.341 | 9.0 × 10−5 2 | [46] |
| FCB group | Marine | Flavobacterium algicola 12076 | OUC-Jdch16/MW767957.1 | Y | A3 | 6 | 25 | 1043 | 16.251 | 0.016 2 | [122] |
| FCB group | Plant | Spirosoma linguale DSM74 | SlFuc29/ADB37178.1 | Y | A3 | 5 | 50 | 180 ± 42 | 154.4 | 0.88 | [123] |
| Proteobacteria | Plant | Xanthomonas campestris pv.campestris str. ATCC 33913 | NixE/AAM42160.1 | Y | A | 5 | 37 | 700 ± 100 | 6.1 ± 2.0 | 8.7 × 10−3 2 | [117] |
| Proteobacteria | Marine | Paraglaciecola sp. | Fp231/MW623630.1 | Y | A | 5.6–6.0 | 25 | 140 ± 10 | 31 ± 0.5 | 0.221 | [57] |
| Proteobacteria | Marine | Vibrio sp. strain EJY3 | VejFCD/AEX22740.1 | N | A3 | – | – | 6700 ± 500 | 4.6 ± 1.4 | 6.9 × 10−4 2 | [37] |
| PVC group | Gut | Akkermansia muciniphila MucT (ATCC BAA-835) | Amuc_0010/ACD03857.1 | Y | A3 | 5.6 | – | 841.23 ± 46.72 | 378.33 | 0.45 | [75] |
| Terrabacteria group | Gut | Bifidobacterium longum subsp. infantis ATCC 15697 | Blon_2336/ACJ53394.1 | N | B | 6–7.5 | 37 | 709 ± 149 | 0.285 ± 0.024 | (407.73 ± 51.34) × 10−6 | [63] |
| Terrabacteria group | Gut | Bifidobacterium longum subsp. infantis ATCC 15697 | Blon_0248/ACJ51376.1 | N | A | 6–7.5 | 37 | 131 ± 10 | 0.110 ± 0.026 | (833.31 ± 134.64) × 10−6 | [63] |
| Terrabacteria group | Gut | Bifidobacterium longum subsp. infantis ATCC 15697 | Blon_0426/ACJ51546.1 | N | A | 6–7.5 | 37 | 180 ± 30 | 4.481 ± 0.329 | (24.95 ± 1.69) × 10−3 | [63] |
| Terrabacteria group | Streptosporangium roseum | SrFucNaFLD/ACZ87343.1 | Y | A | 5.6–7.5 | 37 | 10.59 ± 2.64 | 0.104 ± 0.0261 | 9.8 × 10−3 2 | [118] | |
| Terrabacteria group | Gut | Lactobacillus casei BL23 | AlfA/CAQ67115.1 | N | A | 7.5 | 39 | 270 | 0.8551 | 3.2 × 10−3 2 | [119] |
| Terrabacteria group | Gut | Lactobacillus casei BL23 | AlfB/CAQ67877.1 | N | A | 7 | 41 | 2900 | 4.71 | 1.6 × 10−3 2 | [119] |
| Terrabacteria group | Gut | Lactobacillus casei BL23 | AlfC/CAQ67984.1 | N | A | 7 | 41 | 5200 | 16.281 | 3.1 × 10−3 2 | [119] |
| Terrabacteria group | Hot spring | Paenibacillus sp.3179 | PsFuc/QEX52072.1 | N | A3 | 7.4 | 50 | 1110 ± 750 | (3 ± 1) × 10−3 1 | 2.7 × 10−3 2 | [124] |
| Terrabacteria group | Gut | Ruminococcus gnavus E1 | E1_10125/- | Y | B | 6 | – | 237.9 ± 39.69 | (18 ± 0.88)× 10−4 | 7.61 × 10−3 | [33] |
| Terrabacteria group | Gut | Ruminococcus gnavus ATCC 29149 | ATCC_03833/ WP_004844769.1 | N | A | 6 | – | 179.1 ± 28.77 | 83.6 ± 2.97 | 467 | [33] |
| Thermotogae | Thermotoga maritima | Thma/AAD35394.1 | N | A | 7 | – | 550 ± 30 | 12.6 ± 0.47 | 0.023 ± 0.007 | [74] | |
| Unclassified | Soil | Soil metagenome | Mfuc1/AIC77298.1 | N | A | 7 | – | 110 ± 10 | 1.33 ± 01 | 0.012 2 | [74] |
| Soil | Soil metagenome | Mfuc2/AIC77299.1 | N | A | 7 | – | 140 ± 10 | 1.92 ± 0.081 | 0.014 2 | [74] | |
| Soil | Soil metagenome | Mfuc4/AIC77301.1 | N | A | 7 | – | 71 ± 10 | 0.64 ± 0.021 | 9.0 × 10−3 2 | [74] | |
| Soil | Soil metagenome | Mfuc5/AIC77302.1 | N | A | 7 | – | 1900 ± 40 | 1.58 ± 0.031 | 8.3 × 10−4 2 | [74] | |
| Soil | Soil metagenome | Mfuc6/AIC77303.1 | N | A | 9 | – | 400 ± 50 | 0.47 ± 0.041 | 1.2 × 10−3 2 | [74] | |
| Soil | Soil metagenome | Mfuc7/AIC77304.1 | N | A | 6 | – | 280 ± 50 | 1.83 ± 0.131 | 6.5 × 10−3 2 | [74] |
Note: kinetic parameters were obtained using aryl-Fuc substrates.
1
Estimated from reported Vmax (μmol/L/min/mg) and molecular weight (g/mol, MW) using kcat (s−1) = Vmax × MW/1000/60.
2
Based on kcat /Km.
–, Data unavailable.
3
Predicted based on sequence analysis.
FCB, Fibrobacteres-Chlorobi-Bacteroidetes super phylum; Opt, optimal; PVC, Planctomycetes-Verrucomicrobia-Chlamydiae bacterial superphylum; SP, signal peptide.