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. 2023 Apr 19;14:1177722. doi: 10.3389/fimmu.2023.1177722

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Copyright © 2023 Solé, Parras, Yamanouchi, Garnica, Garabatos, Moro, Montaño, Mondal, Fandos, Yang, Serra and Santamaria

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cell (sc) RNAseq analysis of tetramer+ CD4+ T-cells from pMHCII-NP-treated mice. (A) t-SNE plot of scRNAseq data for tetramer+ cells isolated from mice treated with InsB_12-20-CT-R1/IA^g7-, InsB_13-21-R2/IA^g7- or InsB_10-23-CT-R3/IA^g7-NPs as compared to tetramer– cells (control) purified from InsB_12-20-CT-R1/IA^g7-NP-treated mice. (B) t-SNE plots of scRNAseq data for cells from the mice in (A) plus tetramer+ cells from NOD mice treated with BDC2.5mi/IA^g7-NPs. Left, cluster types; Right, pMHCII specificities. (C) Table depicting the number of cells belonging to each cell cluster as a function of tetramer-binding specificity. (D) Correlation between the levels of gene expression among TR1-like (cluster #2) cells from mice treated with InsB_12-20-CT-R1/IA^g7-NP, InsB_13-21-R2/IA^g7-NP, InsB_10-23-CT-R3/IA^g7-NP and BDC2.5mi/IA^g7-NPs as compared to tetramer– cells (Tconv control) purified from InsB_12-20-CT-R1/IA^g7-NP-treated mice. The P value refers to the Pearson correlation coefficient for each comparison.