FIGURE 4.

Polymerase θ silencing enhances in vitro radiosensitivity in lung cancer. (A) Clonogenic survival assay showing that POLQ silencing enhances radiosensitivity in A549, H1299^WT, and H1299^IRR cells. (B) Neutral comet assay showing the effect of POLQ silencing on tail formation. (C) Representative immunostaining images and quantification showing γH2AX foci 24 h after irradiation (6 Gy). A cell containing ≥10 foci is considered foci‐positive. (D) Cell cycle distribution is detected in A549, H1299^WT, and H1299^IRR cells 24 h after irradiation (6 Gy). (E) POLQ silencing increases radiation‐induced cell apoptosis in A549, H1299^WT, and H1299^IRR cells 48 h after irradiation (6 Gy).