FIGURE 5.

Polymerase θ inhibition with NVB impairs in vitro radioresistance in lung cancer. (A) The effect of NVB on colony formation ability after irradiation in A549, H1299^WT, and H1299^IRR cells. (B) Representative images of neutral comet assay performed 4 h after radiation in lung cancer cells pretreated with NVB or PBS for 24 h. (C) Representative images and quantification of γH2AX foci in A549, H1299^WT, and H1299^IRR cells pretreated with NVB. (D) Cell cycle distribution is examined in NVB‐ or PBS‐treated lung cancer cells 24 h after irradiation (6 Gy). (E) NVB treatment increases radiation‐induced cell apoptosis in A549, H1299^WT, and H1299^IRR cells 48 h after irradiation (6 Gy).