FIGURE 3.

Formin‐like 2 (FMNL2) promotes angiogenesis and metastasis of colorectal cancer (CRC) in vivo. (A, B) Weight of subcutaneous tumor tissues derived from FMNL2‐overexpressed cells and control cells in BALB/c mice. n = 6 for each group. (C, D) Weight of subcutaneous tumor tissues derived from FMNL2‐silenced cells and control cells in BALB/c mice. n = 5 for each group. (E) Representative images of CD31 and FMNL2 expression in an indicated group of paraffin‐embedded subcutaneous tissues by immunohistochemical staining. (F, G) Quantifying microvessel density (MVD) by evaluating CD31 expression in an indicated group of subcutaneous tumor tissues. (H, I) Formation of primary CRC, liver metastases derived from FMNL2‐overexpressed SW480 cells, and control cells in BALB/c mice. Representative gross images of CRC orthotopic tumors and corresponding livers are shown. n = 5 for each group. *p < 0.05. NC, negative control