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. 2023 Feb 24;114(5):2014–2028. doi: 10.1111/cas.15739

FIGURE 4.

FIGURE 4

Formin‐like 2 (FMNL2) interacts with epidermal growth factor‐like protein 6 (EGFL6) in colorectal cancer (CRC) cells. (A) Coimmunoprecipitation (IP) assay of the interaction between FMNL2 and EGFL6. (B) Representative images of immunofluorescence colocalization assay of FMNL2 and EGFL6 in CRC cells. (C) Schematic diagram of different truncations of FMNL2. (D) GST pull‐down assay of the interaction between several truncations of FMNL2 and EGFL6. (E) Expression of EGFL6 in FMNL2‐overexpressed SW480 and DLD‐1 cells, FMNL2‐silenced SW620 and LoVo cells, and control cells by western blot analysis (WB). (F) Expression of FMNL2 in EGFL6‐overexpressed RKO and HCT116 cells, EGFL6‐silenced SW480 and DLD‐1 cells, and control cells by WB. Expression of FMNL2 in EGFL6‐overexpressed RKO and HCT116 cells, EGFL6‐silenced SW480 and DLD‐1 cells, and control cells by WB. NC, negative control