FIGURE 5.

Formin‐like 2 (FMNL2) promotes the paracrine of epidermal growth factor‐like protein 6 (EGFL6) in colorectal cancer (CRC). (A) Expression of EGFL6 protein in the conditioned medium (CM) of FMNL2‐overexpressed SW480 and DLD‐1 cells and FMNL2‐silenced SW620 and LoVo cells by western blot analysis (WB). (B, C) Expression of EGFL6 protein in the CM of FMNL2‐overexpressed SW480 and DLD‐1 cells and FMNL2‐silenced SW620 and LoVo cells by ELISA assay. (D) Morphology of SW480 and DLD‐1 cells was examined by transmission electron microscopy. (E) WB detected the extracellular vesicle (EV) and intracellular expression of the transmembrane protein TSG101 and CD9 in DLD‐1 and SW480 cells, respectively. (F) Immunofluorescence analysis detected the expression and location of CD9 in DLD‐1, SW480, and HCT116 cells with different EGFL6 expressions. Representative images are shown