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. 2023 Feb 24;114(5):2014–2028. doi: 10.1111/cas.15739

FIGURE 6.

FIGURE 6

Epidermal growth factor‐like protein 6 (EGFL6) mediates angiogenesis by formin‐like 2 (FMNL2). (A, B) Transwell assay was carried out in HUVECs treated with conditioned medium (CM) from EGFL6‐overexpressing RKO and HCT116 cells, with or without FMNL2 knockdown, to investigate the effect on the migration ability of HUVECs. (C, D) Transwell migration assay to investigate the effect of CM from EGFL6‐knockdown SW480 and DLD‐1 cells, with or without FMNL2 overexpression, on the migration ability of HUVECs. (E, F) Tube formation to investigate the effect of CM from EGFL6‐overexpressing RKO and HCT116 cells, with or without FMNL2 knockdown, on HUVEC tube formation. (G, H) Tube formation to investigate the effect of CM from EGFL6‐knockdown SW480 and DLD‐1 cells, with or without FMNL2 overexpression, on HUVEC tube formation. (I, J) Chorioallantoic membrane (CAM) assay was used to investigate the effect of CM from EGFL6‐overexpressing RKO and HCT116 cells, with or without FMNL2 knockdown, on HUVEC tube formation. (K, L) CAM assay was carried out to investigate the effect of CM from EGFL6‐knockdown SW480 and DLD‐1 cells with or without FMNL2‐overexpression, on HUVEC tube formation. Data are presented as the mean ± SEM; n = 3 independent experiments. **p < 0.01, ***p < 0.001. NC, negative control