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. 2023 Feb 14;114(5):2001–2013. doi: 10.1111/cas.15741

FIGURE 3.

FIGURE 3

GNB1 overexpression induces epithelial‐to‐mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells and promotes metastasis. (A) Cells migration at 24 h after scratching (0 h) in HLF cells with GNB1 overexpression were determined by wound‐healing assays. The scale bar represents 200 μm. *p < 0.05. (B) Cell migration at 48 h after scratching (0 h) in Huh7 and 97H cells with GNB1 knockdown were determined by wound‐healing assays. The scale bar represents 200 μm. **p < 0.01. (C) Cell migration and invasion abilities in HLF cells with GNB1 overexpression were determined by transwell assay. Representative image (left panel) and summary bar chart (left panel) are shown. Scale bar, 50 μm. ***p < 0.001. (D) Cell migration and invasion abilities in 97H and Huh7 cells with GNB1 knockdown were determined by transwell assay. Representative image (left panel) and summary bar chart (left panel) are shown. Scale bar, 50 μm. ***p < 0.001, Student t test. (E and F) Expression of EMT markers, N‐cadherin and occludin in indicated cells were examined by western blotting.