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. 2023 Feb 14;114(5):2001–2013. doi: 10.1111/cas.15741

FIGURE 4.

FIGURE 4

GNB1 activates the mitogen‐activated protein kinase (MAPK) pathway in hepatocellular carcinoma (HCC) cells. (A) GSEA of the RNA‐seq data revealed significant overlaps among MAPK pathway‐targeted genes. (B) Important molecules in the MAPK pathway, such as p‐ERK, ERK, p‐P38, P38, p‐JNK, and JNK, were determine by western blotting in GNB1 overexpression cell lines. Stable overexpression of GNB1 enhanced the phosphorylation of P38 and ERK in HLF cells. (C) Important molecules in the MAPK pathway levels were determined by western blotting in GNB1 knockdown cell line. Knockdown of GNB1 inhibited the phosphorylation of P38 and ERK in Huh7 and 97H cells. (D) HLF cells stably overexpressing GNB1 were treated with SB202190 (10 μmol/L). Wound‐healing assay was performed to assess cell migrative abilities. SB202190 exposure significantly attenuated the enhancement in cell migration induced by GNB1 overexpression. (E) Transwell assay was performed to assess cell invasive and migrative abilities. SB202190 exposure significantly attenuated the enhancement in cell migration and invasion induced by GNB1 overexpression. (F) Cell viability of indicated cells were measured by CCK‐8 assay. SB202190 treatment reversed the increased proliferation induced by GNB1 overexpression in HLF cells. *p < 0.05; **p < 0.01; ***p < 0.001, Student t test.