FIGURE 4.

Sharing of somatic mutation profiles defined by multiregional sequencing of keratinizing and nonkeratinizing squamous cell carcinomas (SCC) in primary and disseminated tumors. A, Gross findings of the sample collection sites of the primary and disseminated tumors in the Douglas pouch are shown, as well as the microscopic findings of each sample (100× magnification; scale bar, 100 μm). Kera2 and Nonkera1 were in the consecutive area on the same section. B, Sharing pattern of somatic single‐nucleotide variants (SNVs) and short insertions/deletions (Indels) among 10 samples, including five samples in Figure 2. The multiregional analysis in Figure 4 was limited to the mutation sites identified in Figure 2 because formalin‐fixed paraffin‐embedded (FFPE) samples included low‐depth samples. All shared somatic SNVs and Indels and MAF are shown in Table S8