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. 2023 Jan 31;114(5):1929–1942. doi: 10.1111/cas.15726

FIGURE 4.

FIGURE 4

MicroRNA (miR)‐29c‐3p downregulation in cancer‐associated fibroblast‐derived exosomes (CAF‐Exos) promotes metastasis of ovarian cancer in vitro and in vivo. (A) Schematic illustration of the indirect coculture of CAFs transiently transfected with cy3‐tagged miR‐29c‐3p mimics (CAFs‐miR‐29c‐3p‐cy3) with GFP‐labeled ovarian cancer cells SKOV3 (SKOV3‐GFP). (B) The representative immunofluorescence images show the uptake of cy3‐tagged miR‐29c‐3p (red) by SKOV3‐GFP cells (green). Scale bar, 20 μm. (C) Histograms showing the relative expression of miR‐29c‐3p in CAF transfected with miR‐29c‐3p mimics and their exosomes detected by quantitative RT‐PCR (qRT‐PCR). (D) Histograms showing the relative expression of miR‐29c‐3p in SKOV3 cells indirectly cocultured with CAFs transfected with miR‐29c‐3p mimics or negative control (NC) mimics in detected by qRT‐PCR. (E–G) Mice bearing ES2‐luc orthotopic xenotransplants were injected intraperitoneally with PBS, CAF‐NC‐Exo, or CAF‐miR‐29c‐3p‐Exo. (E) Bioluminescence images of abdominal metastasis in mice bearing ES2‐luc intraperitoneal transplanted mouse model injected with PBS, CAF‐NC‐Exo, or CAF‐miR‐29c‐3p‐Exo. (F) Quantitative radiance values from IVIS imaging in metastases on abdominal organs. (G) Quantitative radiance values from IVIS imaging of ES2‐luc intraperitoneal transplanted mouse. (H, I) Histograms showing the number of migration and invasion SKOV3 cells transfected with miR‐29c‐3p mimics, NC mimics, or miR‐29c‐3p inhibitors and NC inhibitors. *p < 0.05; **p < 0.01; ***p < 0.01. ns, no significance