Fig. 3.

²H NMR spectra of the individually ²H-labeled molecules in the Cer[EOS]/Cer[NS24]/FFA-mix/Chol mixture (0.3/0.7/1/0.45 M ratio) at varying temperatures hydrated with 50 wt% buffer. From left to right, the figure shows the ²H NMR spectra of the deuterated acyl chain of Cer[NS]-d₄₇, the deuterated chain of LA-d₄₇ of the FFA mix, and the partially deuterated Chol-d₆ in the right column. The bar plots on the far right report the phase proportions derived from the numerical simulations of the ²H NMR line shapes (red lines). The ²H NMR spectra were acquired with a repetition time of 50 s to achieve full relaxation of all components, allowing quantification. LA, lignoceric acid.